听力障碍青少年治疗过程心理干预对患儿语言能力恢复和心境状态的影响

摘要 目的：探讨听力障碍青少年治疗过程心理干预对患儿语言能力恢复和心境状态的影响。方法：选取我院2019年6月到2020年6月共收治的80例7~18岁听力障碍青少年作为研究对象,将患儿随机分为观察组与对照组,每组40例。对所有患儿应用人工耳蜗植入术进行治疗,给予对照组常规言语训练以及健康教育等干预。观察组患儿在常规干预基础上增加全治疗过程的心理干预。对比两组患儿的治疗效果,语言能力恢复,心境状态,ABC评分。结果：观察组患儿治疗的总有效率为92.50%,对照组患儿治疗的总有效率为75.00%,观察组高于对照组（P＜0.05）;两组患儿干预前的言语形成、听觉能力以及听觉感知对比无明显差异（P＞0.05）,两组患儿通过治疗与干预之后,言语形成、听觉能力以及听觉感知明显提升,且观察组评分高于对照组（P＜0.05）;两组患儿干预前的精神、控制、能力、忍受消极情感以及接受变化对比无明显差异（P＞0.05）,两组患儿通过治疗与干预之后,精神、控制、能力、忍受消极情感以及接受变化明显降低,且观察组评分低于对照组（P＜0.05）;两组干预前的兴奋、易激怒、刻板行为以及不恰当语言等评分比较无统计学意义（P＞0.05）,两组患儿干预后,各评分均下降,其中观察组小于对照组,差异有统计学意义（P＜0.05）。结论：对听力障碍青少年在常规治疗与干预基础上增加心理干预,能够提升患儿的治疗效果,提升语言能力恢复情况,减少负面情绪,提升患儿的心境状态,值得临床应用推广。     

冠状动脉支架植入患者心理弹性状况调查及与生活质量的关系研究

目的:调查冠状动脉支架植入(ICS)患者心理弹性状况,并分析心理弹性与生活质量的关系。方法:选取2019年3月~2021年4月我院收治的ICS患者120例作为观察组,另选取同期来我院进行健康体检的志愿者80例作为对照组,对比两组患者的生活质量评分,以心理弹性量表(CD-RICS)调查ICS患者的心理弹性状况,采用Pearson相关分析ICS患者生活质量与心理弹性的相关性,采用Logistic回归分析ICS患者心理弹性水平的影响因素。结果:观察组36条目健康调查简表(SF-36)各维度评分及总评分均低于对照组(P<0.05)。120例ICS患者的心理弹性总分为(64.41±5.73)分,心理弹性各维度得分由低到高分别为乐观、力量、坚韧。Pearson相关分析结果表明,ICS患者的生活质量平均分与心理弹性总分呈正相关(P<0.05)。冠心病病程、文化程度、家庭月收入、兴趣爱好数量、居住地对ICS患者的心理弹性得分有影响(P<0.05)。冠心病患病时间≥10年、文化程度为初中及其以下、家庭月收入≤3000元、兴趣爱好为1~2个、居住地为农村是ICS患者心理弹性水平的影响因素(P<0.05)。结论:ICS患者的心理弹性水平一般,且可影响患者生活质量,受到冠心病病程时长、文化程度、家庭收入水平、兴趣爱好、居住地等多种因素影响,应制定合理措施来提高ICS患者的心理弹性,促进患者身心健康。     

Transcriptome analysis of the endometrium from Chinese Erhualian sows that differ in calcium ion concentration and litter size

Embryonic survival rate, an important factor in the fecundity of sows, is affected by endometrium‐secreting histotroph. A higher concentration of calcium ion has been observed in the uterus of highly prolific Erhualian sows (EH) compared with those of less prolific (EL) sows. This suggests that EH sows have better establishment and maintenance of pregnancies, thus increasing embryonic survival rate during the peri‐implantation period. To understand the mechanisms of how the endometrium‐secreting histotroph affects embryonic survival rate during the Erhualian peri‐implantation period, the expression patterns of endometrial mRNA in the EH and EL sows on day 12 of gestation were analyzed using RNA sequencing technology. A total of 164 differentially expressed genes (DEGs) were identified (P_adj < 0.05, |log₂(FC)| ≥ 1), including 46 upregulated and 118 downregulated genes in EH compared to EL. Gene Ontology enrichment indicated that a subset of DEGs was involved in calcium ion binding and cell adhesion. Solute carrier family 8 member A3 and solute carrier family 24 member 4, identified as upregulated genes (P_adj < 0.05) in EH, were considered key candidate genes expressed in the endometrium affecting embryonic survival rate during the peri‐implantation period. The results improve understanding of the genetic mechanism underlying the variation in litter size of Erhualian pigs during the peri‐implantation period.     

Calcitonin administration improves endometrial receptivity via regulation of LIF,Muc-1 and microRNA Let-7a in mice

Calcitonin (CT) is one of the factors affecting the embryo implantation, but its effects on the implantation window have not been fully investigated. The current study investigated the effects of CT on the endometrium receptivity by morphological study and evaluation of leukemia inhibitory factor (LIF), mucin 1 (Muc-1), and microRNA (miRNA) Let-7a in the ovarian stimulation and the normal ovarian cycle. Then the mechanism of the CT effects through the mammalian target of rapamycin (mTOR) signaling pathway was studied by using PP242. A total of 64 BALB/c mice were divided into the normal ovarian cycle and ovarian stimulation groups. Each group consisted of four subgroups: control, calcitonin, PP242, and calcitonin+PP242. CT and PP242 were injected on the fourth of pregnancy into the mice and 24 hr later all the mice were killed. The uterine tissue samples were used for morphological analysis, and endometrial cells were mechanically isolated for evaluation of gene and protein expression. The results showed that ovarian stimulation induced mTOR phosphorylation as well as increased expression of the Let-7a miRNA. In addition, CT injection increased the expression of LIF and miRNA Let-7a in ovarian stimulation similar to that in normal ovarian cycles. However, injection of PP242 reduced expression of miRNA Let-7a and increased Muc-1 expression in ovarian stimulation group. In conclusion, the administration of CT improved endometrial receptivity in mice. This phenomenon occurred by upregulation of LIF, miRNA Let-7a and downregulation of Muc-1 via mTOR signaling pathway.     

转基因受体酵母菌的低能氩离子注入研究

通过不同剂量的低能Ar 注入酿酒酵母Ke-y,筛选出了一种较好的菌体保护液,获得了一系列Ar 注入参数。在该研究体系内,Ar 最佳注入剂量分别为9 0×1015 ions/cm2 或13 5×1015 ions/cm2(适于IBB Device 1 型离子注入机)和1 0×1016ions/cm2 或1 4×1016 ions/cm2(适于LZD1000型离子注入机),为离子束介导外源基因组DNA在酵母菌Ke-y中的遗传转化奠定了基础。     

Delayed luminescence of Prorocentrum minimum under controlled conditions

Delayed luminescence (DL), also termed delayed fluorescence or delayed light emission, is the phenomenon of long-lived light emission by plants and cyanobacteria after being illuminated with light and put into darkness. Culture growth of three Prorocentrum minimum strains was studied with DL measurements. DL decay kinetics was measured from 1–60 s after a pulse of white light. The strains used were from the Adriatic Sea (PmK), from Chesapeake Bay, USA (D5), and from the Baltic Sea (BAL), cultured at salinity of 32, 16, and 8 (practical salinity scale), respectively. The strains differed in cell size and chlorophyll a content (PmK > D5 > BAL), as well as in DL parameters. The DL results were compared to standard measurements of culture density and carbon content (calculated from biovolumes). DL decay curves had a specific peak, which changed with culture growth and showed more similarities between the strains PmK and D5. The DL intensity increased with cell density and carbon content in a two-stage process, corresponding to the lag and exponential phases of growth. DL intensity was best correlated with carbon content irrespective of strain and is proposed as an estimate of biomass and for differentiating between lag and exponential phases of growth.     

低能铁离子束对原卟啉IX二钠盐的辐照效应研究

110ke V Fe^ 离子注入原卟啉IX二钠盐薄膜亲品后的一些谱学分析结果表明,低能铁离子束辐照可以导致生物分子的损伤和化学改性,并且初步证实注入铁离子在样品分子中慢化沉积后形成含铁的金属络合物,即注入铁离子的质量沉积。     

低能铁离子束辐照氨基酸衍生物的红外光谱结构表征

利用傅里叶变换红外光谱（FTIR）技术研究了能量为110keV的Fe^ 离子束辐照L（ ）-半胱氨酸盐酸盐单晶水合物固态样品的结构变化情况。对辐照样品的两次红外光谱分析结果有所不同,表明固态样品不同部位受低能铁离子束作用后可发生不同的分子改性;但二者又具有一定的相似性,都反映了原分子中氨基、羧基和巯基等基团的受损及硝基和酰胺基团等的生成。     

离子束注入对衣康酸生产菌种的改良

用10keV、剂量5.2×10~(14)～5.2×10~(15)ions/cm~2的氮离子注入衣康酸生产菌株土曲霉A9003,在2％LiCl抗性平板筛选到一株能在39℃发酵的衣康酸高产菌株。该菌株在30L发酵罐发酵产酸7.5％,转化率60.1％,发酵周期50h。